Curated Data based on Instance of Characterization
As Received
 
Nanomaterial State:
liquid suspension
Manufacturer:
Nanocomposix
  • Physico-Chemical Characteristics
  • biological interactions
  • Environmental interactions
Particle Size

Mean Diameter:
47.5 nm +/- 24.6 nm
Transmission Electron Microscopy
Diameter:
7.3 - 125.8 nm
Transmission Electron Microscopy
Mean Hydrodynamic Diameter:
215 nm +/- 99 nm
Dynamic Light Scattering
Composition
graph
CORE (hover over circle for proportion of material)
arrow Proportion of Nanomaterial:    Not reported
Component 1
Material Type:
metal
Molecular Identity:
Al

COATING (hover over circle for proportion of material)
arrow Proportion of Nanomaterial:    Not reported
Component 1
Molecular Identity:
aluminum oxide
Surface Charge

Zeta Potential:
35 mV
Electrophoretic Light Scattering

In Vitro Study 1; Cytotoxicity; MTT Assay

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: neonatal HEK
Cell Source: Lonza Walkersville Inc.
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: cytotoxicity
Assay Used:
Assay Name: MTT assay
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 0.4, 0.04, 0.004, 0.0004 mg/mL
Duration of Exposure / Incubation Time: 24 hours
Endpoint
Toxicity:
Cytotoxicity: Detected Quantitatively

In Vitro Study 2; Cytokines

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: neonatal HEK
Cell Source: Lonza Walkersville Inc.
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: Florescent Labeled Cytokines
Assay Used:
Manufacturer: Bio-Rad Laboratories
Assay Name: Bio-Plex suspension array system
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 0.4, 0.04, 0.004, 0.0004 mg/mL
Duration of Exposure / Incubation Time: 24 hours
Endpoint
Mechanistic Analysis:
Protein Expression (IL-8,ILl-6, IL-1B, IL-10, and TNF-a): Detected Quantitatively

In Vitro Study 3; Uptake

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: neonatal HEK
Cell Source: Lonza Walkersville Inc.
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: TEM/EDS
Assay Used:
Assay Name: Trump's fixative
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 0.4, 0.04, 0.004, 0.0004 mg/mL
Duration of Exposure / Incubation Time: 24 hours
Endpoint
Cellular Uptake:
Cellular Uptake 1: Detected Qualitatively

In Vitro Study 4; Cytotoxicity; AQueous One (96AQ)

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: neonatal HEK
Cell Source: Lonza Walkersville Inc.
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: cytotoxicity
Assay Used:
Assay Name: AQueous One (96AQ)
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 0.4, 0.04, 0.004, 0.0004 mg/mL
Duration of Exposure / Incubation Time: 24 hours
Endpoint
Toxicity:
Cytotoxicity: Detected Quantitatively

In Vitro Study 5; Cytotoxicity; Alamar Blue (aB)

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: neonatal HEK
Cell Source: Lonza Walkersville Inc.
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: cytotoxicity
Assay Used:
Assay Name: Alamar Blue (aB)
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 0.4, 0.04, 0.004, 0.0004 mg/mL
Duration of Exposure / Incubation Time: 24 hours
Endpoint
Toxicity:
Cytotoxicity: Detected Quantitatively
As Processed A
 
Time Point:
0 hours
Nanomaterial State:
liquid suspension
Manufacturer:
Nanocomposix
Processing Type:
suspended
  • Physico-Chemical Characteristics
  • biological interactions
  • Environmental interactions
Particle Size

Mean Hydrodynamic Diameter:
325.8 nm +/- 12.6 nm
Dynamic Light Scattering
Mean Hydrodynamic Diameter:
263.8 nm +/- 11.2 nm
Dynamic Light Scattering
As Processed B
 
Time Point:
0 hours
Nanomaterial State:
liquid suspension
Manufacturer:
Nanocomposix
Processing Type:
suspended
  • Physico-Chemical Characteristics
  • biological interactions
  • Environmental interactions
Particle Size

Mean Hydrodynamic Diameter:
3217.5 nm +/- 130.5 nm
Dynamic Light Scattering
Mean Hydrodynamic Diameter:
7893 nm +/- 27 nm
Dynamic Light Scattering
As Processed C
 
Time Point:
24 hours
Predecessor:
As Processed A, 0 hours
Nanomaterial State:
liquid suspension
Manufacturer:
Nanocomposix
Processing Type:
suspended
  • Physico-Chemical Characteristics
  • biological interactions
  • Environmental interactions
Particle Size

Mean Hydrodynamic Diameter:
615.5 nm +/- 18.0 nm
Dynamic Light Scattering
Mean Hydrodynamic Diameter:
327.6 nm +/- 14.7 nm
Dynamic Light Scattering
As Processed D
 
Time Point:
24 hours
Predecessor:
As Processed B, 0 hours
Nanomaterial State:
liquid suspension
Manufacturer:
Nanocomposix
Processing Type:
suspended
  • Physico-Chemical Characteristics
  • biological interactions
  • Environmental interactions
Particle Size

Mean Hydrodynamic Diameter:
3725.5 nm +/- 214.5 nm
Dynamic Light Scattering
Mean Hydrodynamic Diameter:
4741 nm +/- 812 nm
Dynamic Light Scattering

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MIAN-specific data are curated from data sources and presented on the nanomaterial details page, below. When available, the original publication link is provided so that you can verify the source data and access data that is outside of the NR MIAN. The NR Descriptor is a high level classification of the nanomaterial based on primary composition and shape.
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