Curated Data based on Instance of Characterization
As Synthesized
 
Nanomaterial State:
liquid suspension
Synthesis Method:
FSP/suspension in BSA-containing media
  • Physico-Chemical Characteristics
  • biological interactions
  • Environmental interactions
Composition
graph
CORE (hover over circle for proportion of material)
arrow Proportion of Nanomaterial:    Not reported
Component 1
Molecular Identity:
TiO2
Material Type:
metal oxide
Component 2
Molecular Identity:
bovine serum albumin
Material Type:
Biological Type:

protein
As Processed A
 
Nanomaterial State:
liquid suspension
Processing Type:
suspended
  • Physico-Chemical Characteristics
  • biological interactions
  • Environmental interactions
Particle Size

Mean Hydrodynamic Diameter:
Intensity Weighted:
131.5 nm
Dynamic Light Scattering
Surface Charge

Zeta Potential:
-9.58 mV
Electrophoretic Light Scattering

In Vitro Study 1; Dose Response

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: BEAS-2B
Strain: CRL- 9609
Cell Source: ATCC
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: Fluorescence microscopy
Assay Used:
Cocktail mix 1: Hoechst 33342 (1 uM) and JC1 (1 uM)
Cocktail mix 2: Hoechst 33342 (1 uM), Fluo-4 (5 uM), and propidium iodide (5 uM)
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 6, 13, 25, 50 µg/mL
Duration of Exposure / Incubation Time: 3 hours
Duration Between Exposure and Measurement: 30 minutes
Endpoint
Toxicity:
Cytotoxicity: Reported Qualitatively

In Vitro Study 2; Time Response

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: BEAS-2B
Strain: CRL-9609
Cell Source: ATCC
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: Fluorescence microscopy
Assay Used:
Cocktail mix 1: Hoechst 33342 (1 uM) and JC1 (1 uM)
Cocktail mix 2: Hoechst 33342 (1 uM), Fluo-4 (5 uM), and propidium iodide (5 uM)
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 50 µg/mL
Duration of Exposure / Incubation Time: 1, 2, 3, 6 hours
Duration Between Exposure and Measurement: 30 minutes
Endpoint
Toxicity:
Cytotoxicity: Reported Qualitatively
As Processed B
 
Nanomaterial State:
liquid suspension
Processing Type:
suspended
  • Physico-Chemical Characteristics
  • biological interactions
  • Environmental interactions
Particle Size

Mean Hydrodynamic Diameter:
Intensity Weighted:
115.1 nm
Dynamic Light Scattering
Surface Charge

Zeta Potential:
-9.95 mV
Dynamic Light Scattering

In Vitro Study 1; Dose Response

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: RAW 624.7
Strain: TIB71
Cell Source: ATCC
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: Fluorescence microscopy
Assay Used:
Cocktail mix 1: Hoechst 33342 (1 uM) and JC1 (1 uM)
Cocktail mix 2: Hoechst 33342 (1 uM), Fluo-4 (5 uM), and propidium iodide (5 uM)
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 6.25, 12.5, 25, 50 µg/mL
Duration of Exposure / Incubation Time: 3 hours
Duration Between Exposure and Measurement: 30 minutes
Endpoint
Toxicity:
Cytotoxicity: Reported Qualitatively

In Vitro Study 2; Time Response

Test Subject
Test Subject 1:
Cell Culture Type: cultured cell line
Name: RAW 264.7
Strain: TIB71
Cell Source: ATCC
General Study Details
General:
Test Location: laboratory
Controls Used/Reported: Negative
Standard Protocol Used: Fluorescence microscopy
Assay Used:
Cocktail mix 1: Hoechst 33342 (1 uM) and JC1 (1 uM)
Cocktail mix 2: Hoechst 33342 (1 uM), Fluo-4 (5 uM), and propidium iodide (5 uM)
Exposure Summary
General:
Method of Exposure: inoculation
Acute or Chronic Exposure: Acute exposure
Intended Dose: 50 µg/mL
Duration of Exposure / Incubation Time: 1, 2, 3, 6 hours
Duration Between Exposure and Measurement: 30 minutes
Endpoint
Toxicity:
Cytotoxicity: Reported Qualitatively

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MIAN-specific data are curated from data sources and presented on the nanomaterial details page, below. When available, the original publication link is provided so that you can verify the source data and access data that is outside of the NR MIAN. The NR Descriptor is a high level classification of the nanomaterial based on primary composition and shape.
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